UV-Visible Spectroscopy

 

🌈 Quantitative Analysis using UV-Visible Spectroscopy
UV-Visible Spectroscopy is one of the most commonly used analytical techniques for both qualitative and quantitative analysis of compounds.
⚙️ Principle
This technique is based on the absorption of ultraviolet or visible light by molecules, causing electronic transitions. The amount of light absorbed is proportional to the concentration of the analyte, following Beer-Lambert’s Law.
🧪 My Practical Exposure
I worked on:
✔ Preparation of standard and sample solutions
✔ Measuring absorbance at specific wavelengths
✔ Constructing calibration curves
✔ Determining unknown concentrations
📊 Applications
✔ Quantitative estimation of compounds
✔ Reaction monitoring
✔ Dye and pollutant analysis
✔ Pharmaceutical analysis
✨ Key Insight
UV-Vis spectroscopy enhanced my understanding of how light absorption can be used for precise quantitative measurements.